Journal: Communications chemistry

Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance.

doi: 10.1038/s42004-022-00661-z

Figure Lengend Snippet: Fig. 4 Impact of BSO-OxOAc as compared to oxaliplatin and cisplatin on the viability of the indicated cancer cell lines and their sublines with the respective acquired platinum drug resistance. a Impact of a 72 h continuous drug exposure on the viability of human colon cancer cells HCT116 and a oxaliplatin-resistant subline HCT116/OxR, as well as the human ovarian cancer cell model A2780 and the cisplatin-resistant subline A2780/Cis. One representative experiment out of at least three performed in triplicate is shown. Data points are depicted as mean ± SD. b IC50 values were derived from the dose-response curves as shown under (a) using the four-parameter logistic nonlinear regression model. Resistance factors were calculated by dividing the IC50 values for the resistant subline by those of the respective sensitive parental cell model.

Article Snippet: The human and murine colorectal carcinoma cell lines HCT116 (Expasy accession: CVCL_0291) and CT26 (CVCL_7254), respectively, were obtained from the American Type Culture Collection (ATCC) (Rockville, MD, USA), and the human ovarian cancer cell line A2780 (CVCL_0134, product number 93112519) together with its cisplatin-resistant subline A2780/Cis (93112517) from Sigma Aldrich.

Techniques: Derivative Assay

Journal: Communications chemistry

Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance.

doi: 10.1038/s42004-022-00661-z

Figure Lengend Snippet: Fig. 5 Cellular uptake of BSO-OxOAc as compared to free oxaliplatin and impact of acquired oxaliplatin resistance. a Parental HCT116 and oxaliplatin- resistant HCT116/OxR cells were exposed to the indicated concentrations of BSO-OxOAc and oxaliplatin for 3 h and cellular uptake quantified by ICP-MS. Data are depicted as mean ± SD. b Log-fold difference in the platinum accumulation between parental HCT116 and HCT116/OxR calculated from the data shown in (a). Statistical significance was tested using one-way ANOVA. *p < 0.05; ***p < 0.001.

Article Snippet: The human and murine colorectal carcinoma cell lines HCT116 (Expasy accession: CVCL_0291) and CT26 (CVCL_7254), respectively, were obtained from the American Type Culture Collection (ATCC) (Rockville, MD, USA), and the human ovarian cancer cell line A2780 (CVCL_0134, product number 93112519) together with its cisplatin-resistant subline A2780/Cis (93112517) from Sigma Aldrich.

Techniques:

Journal: Communications chemistry

Article Title: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance.

doi: 10.1038/s42004-022-00661-z

Figure Lengend Snippet: Fig. 6 Impact of BSO-OxOAc as single agent and in combination with the reducing agents NAC (100 µM) and AA (50 µM) on the clonogenic potential of HCT116 cells and the subline with acquired oxaliplatin resistance (HCT116/OxR). Sparsely seeded cells (1 × 103/24-well plate well) were exposed for 10 days to the indicated compounds and derived cell clones stained with crystal violet, photographed, and results evaluated by ImageJ software as described in the Methods section. One respective experiment out of three performed in duplicate is shown under (a) and the respective evaluation under (b), depicted as mean ± SD. Statistical significance was tested using two-way ANOVA. In all cases: *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The human and murine colorectal carcinoma cell lines HCT116 (Expasy accession: CVCL_0291) and CT26 (CVCL_7254), respectively, were obtained from the American Type Culture Collection (ATCC) (Rockville, MD, USA), and the human ovarian cancer cell line A2780 (CVCL_0134, product number 93112519) together with its cisplatin-resistant subline A2780/Cis (93112517) from Sigma Aldrich.

Techniques: Derivative Assay, Clone Assay, Staining, Software

Journal: Metal-Based Drugs

Article Title: Synthesis, Characterization, and In Vitro Photodynamic Activity of Novel Amphiphilic Zinc(II) Phthalocyanines Bearing Oxyethylene-Rich Substituents

doi: 10.1155/2008/284691

Figure Lengend Snippet: Effects of 4 (triangles), 5 (stars), and 8 (squares) on (a) HT29 and (b) HepG2 in the absence (closed symbols) and presence (open symbols) of light ( λ > 610 nm, 40 mW cm -2 , 48 J cm -2 ). Data are expressed as mean value ± SEM of three independent experiments, each performed in quadruplicate.

Article Snippet: The HT29 human colorectal carcinoma cells (from ATCC, no. HTB-38) were maintained in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, cat no. 10313-021) supplemented with fetal calf serum (10%), penicillin-streptomycin (100 units mL -1 and 100 mgmL -1 , resp.), L-glutamine (2 mM), and transferrin (10 mgmL -1 ).

Techniques:

Journal: Metal-Based Drugs

Article Title: Synthesis, Characterization, and In Vitro Photodynamic Activity of Novel Amphiphilic Zinc(II) Phthalocyanines Bearing Oxyethylene-Rich Substituents

doi: 10.1155/2008/284691

Figure Lengend Snippet: Comparison of the IC 50 values (a) of phthalocyanines 4 , 5 , and 8 against HT29 and HepG2.

Article Snippet: The HT29 human colorectal carcinoma cells (from ATCC, no. HTB-38) were maintained in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, cat no. 10313-021) supplemented with fetal calf serum (10%), penicillin-streptomycin (100 units mL -1 and 100 mgmL -1 , resp.), L-glutamine (2 mM), and transferrin (10 mgmL -1 ).

Techniques: Comparison

Journal: American Journal of Cancer Research

Article Title: CYR61 promotes colorectal carcinoma progression via activating epithelial-mesenchymal transition

doi:

Figure Lengend Snippet: CYR61 promotes CRC cell migration and invasion. CYR61 was silenced in SW480 and overexpressed in HT29 cells. For the purpose of silencing, SW480 cells were transfected with CYR61 siRNA or non-targeting siRNA as a control. Whereas HT29 cells were transfected with CYR61- pcDNA3.1 + to upregulate the expression of CYR61 or pcDNA3.1 + empty vector as a control. A-C. Transwell cell migration and invasion assays (Magnification; 100×) revealed that knockdown of CYR61 significantly suppressed the migratory and invasive ability of SW480 cells. D-F. Conversely, overexpressed CYR61 cells exhibited significantly enhanced migratory and invasive abilities of HT29 cells. G and H. The results of the wound healing assay (Scale; 100 µm) revealed that CYR61 knockdown significantly inhibited gap closure in SW480 cells. I and J. Whereas CYR61 overexpression promoted gap closure compared with the control group. The scale bar was 100 μm. Data are presented as standard errors of the mean (**P≤0.008; ***P<0.0008). K and L. Silencing and overexpression of CYR61 was confirmed by Western blot. The results of three representative experiments are shown.

Article Snippet: Cell culture Human colorectal cancer cell lines SW480, HT29, and HCT116 were purchased from the American Type Culture Collection (ATCC).

Techniques: Migration, Transfection, Control, Expressing, Plasmid Preparation, Knockdown, Wound Healing Assay, Over Expression, Western Blot

Journal: American Journal of Cancer Research

Article Title: CYR61 promotes colorectal carcinoma progression via activating epithelial-mesenchymal transition

doi:

Figure Lengend Snippet: CYR61 expression promotes colorectal cancer cell proliferation. To downregulate or upregulate the expression of CYR61 in CRC cells, SW480 cells were transfected either with CYR61 siRNA or Non-targeting siRNA as a control group, whereas HT 29 cells were transfected with CYR61- pcDNA3.1 + or pcDNA3.1 + empty vector. Proliferation of transfected cells was measured using MTT assay and the absorbance read at 570 nm. A. Knockdown of CYR1 significantly suppressed SW480 cell proliferation. B. The proliferation of HT29 cells was significantly increased in response to CYR61 overexpression. C-F. Transfection efficiency was confirmed by RT-PCR and Western blot analysis. Data are presented as standard errors of the mean (*P<0.02; **P≤0.009; and ***P=0.0001). The results of three representative experiments are shown.

Article Snippet: Cell culture Human colorectal cancer cell lines SW480, HT29, and HCT116 were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, MTT Assay, Knockdown, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: American Journal of Cancer Research

Article Title: CYR61 promotes colorectal carcinoma progression via activating epithelial-mesenchymal transition

doi:

Figure Lengend Snippet: Effect of CYR61 silencing on SW480 cell apoptosis. caspase 3 and 9 activities in SW480 transfected cells were measured by spectrophotometry. The activity of caspases in control cells was designated as 1, and the activity values of the study group were standardized against it. A. CYR61 siRNA cells showed a significant increase in the activity of both caspase-3 and caspase-9. B. The expression level of apoptosis-related proteins was detected by western blot. C. The relative expression of proteins was quantified using Image J software by normalizing the intensity of protein bands to tubulin. Standard errors of the mean for three experiments are represented by bar graphs (**P≤0.006; ***P≤0.0005 and ****P<0.0001).

Article Snippet: Cell culture Human colorectal cancer cell lines SW480, HT29, and HCT116 were purchased from the American Type Culture Collection (ATCC).

Techniques: Transfection, Spectrophotometry, Activity Assay, Control, Expressing, Western Blot, Software

Journal: Oncotarget

Article Title: MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42

doi: 10.18632/oncotarget.12704

Figure Lengend Snippet: A. Expression of miR-384 in six CRC cell lines and one normal colorectal mucosa cell line FHC were detected by real-time PCR. B. Over-expression of miR-384 in SW480 and HCT116 CRC cells verified by real-time PCR. C. Wound-healing assay. Histograms represent the average migrated distances at the indicated times. Error bars represent mean±s.d. from three independent experiments. D-E. The migratory and invasive properties of SW480/Vector, SW480/miR-384 and HCT116/Vector, HCT116/miR-384 cells were analyzed using Boyden chambers or Matrigel-coated Boyden chambers. Error bars represent mean±s.d. from three independent experiments. F. Three-dimensional morphologies assay. Histograms represent the average number of filopodia formed by each cell sphere from three independent experiments. Error bars represent mean±s.d.. G. Representative images of gross specimens of liver metastatic lesions formed in mice injected intrasplenically with SW480/Vector and SW480/miR-384. H. The number of liver visible metastatic nodules was shown ( ** p <0.01). (I) Overall survival of mice bearing liver metastases of SW480/Vector and SW480/miR-384 (log-rank test, n=8, ** p <0.01).

Article Snippet: The human CRC cell lines SW480, SW620, SW837, HCT116, HCT15, LOVO and normal colorectal mucosa cell FHC were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Over Expression, Wound Healing Assay, Plasmid Preparation, Injection

Journal: Cell & Bioscience

Article Title: The role of GLI1 for 5-Fu resistance in colorectal cancer

doi: 10.1186/s13578-017-0145-7

Figure Lengend Snippet: Characterization of 5-FU resistant LoVo-R cells. a Shows morphology of LoVo and LoVo-R cells. b Shows the IC50 dose of 5-FU calculated from measurement of cell viability in different concentrations of 5-FU (48 h). The X-axis is 5-FU concentration (μg/ml), and the Y-axis is O.D. values. Significant difference was indicated by *p < 0.05, **p < 0.005, or ***p < 0.0005

Article Snippet: The human colorectal cancer cell line LoVo was purchased from ATCC.

Techniques: Concentration Assay

Journal: Cell & Bioscience

Article Title: The role of GLI1 for 5-Fu resistance in colorectal cancer

doi: 10.1186/s13578-017-0145-7

Figure Lengend Snippet: Up-regulated of GLI1 signaling axis in LoVo-R (in comparison with LoVo) cells. After next generation sequencing, we performed ingenuity pathway analysis (IPA). a Shows up-regulation of GLI1 and its signaling molecules, and the up-regulated genes are in red . b Detection of GLI1 protein in LoVo and LoVo-R cells. β-actin was used as the internal control

Article Snippet: The human colorectal cancer cell line LoVo was purchased from ATCC.

Techniques: Comparison, Next-Generation Sequencing, Control

Journal: Cell & Bioscience

Article Title: The role of GLI1 for 5-Fu resistance in colorectal cancer

doi: 10.1186/s13578-017-0145-7

Figure Lengend Snippet: The effect of GLI1/2 knockdown on 5-FU response in LoVo-R cells. a Real-time PCR detection of GLI1 after GLI1 shRNA expression. b Detection of GLI1 protein by Western blotting. c The effect of GLI1 -ShRNAs (shown as shGLI1) on the IC50 of 5-FU (measured as shown in Fig. b). d Effects of GLI2 shRNAs (shown as shGLI2) on 5-FU response. Significant difference was indicated by *p < 0.05, **p < 0.005, or ***p < 0.0005

Article Snippet: The human colorectal cancer cell line LoVo was purchased from ATCC.

Techniques: Knockdown, Real-time Polymerase Chain Reaction, shRNA, Expressing, Western Blot

Journal: Cell & Bioscience

Article Title: The role of GLI1 for 5-Fu resistance in colorectal cancer

doi: 10.1186/s13578-017-0145-7

Figure Lengend Snippet: The effects of GLI1 knockdown on gene/protein expression. a Real-time analysis of GLI1, GLI2, Snai1 and Snai2 in GLI2 shRNAs-expressing LoVo-R cells. Significant difference was indicated by ***p < 0.0005. b Effects of Gli2 shRNAs on vimentin expression. c The effect of GLI1 knockdown on Sox2 and CD44 proteins

Article Snippet: The human colorectal cancer cell line LoVo was purchased from ATCC.

Techniques: Knockdown, Expressing

Journal: Cell & Bioscience

Article Title: The role of GLI1 for 5-Fu resistance in colorectal cancer

doi: 10.1186/s13578-017-0145-7

Figure Lengend Snippet: Cell invasiveness assay. Different cells were subjected to cell invasiveness as described in the methods, and invasive cells were visualized by blue in the staining ( a ). b Shows the summary from three independent experiments, with the value (number of invasive cells) from LoVo cells as 100

Article Snippet: The human colorectal cancer cell line LoVo was purchased from ATCC.

Techniques: Staining

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: (A) The mRNA expression level of GPR116 in colon cancer and matched normal colon tissue derived from the Renji cohort was detected by real-time quantitative PCR. Error bars in the scatter plots represent SE. (B) Western blots showed GPR116 protein level in six paired colon cancer tissues (T) and adjacent normal colonic tissue (N) of patients from the Renji cohort and the relative GPR116 protein expression. GPR116 expression in Skrzypczak Colorectal (C) Skrzypczak Colorectal 2 (D) and Gaedcke Colorectal (E) grouped by normal colorectal tissue (1) and colorectal carcinoma (2) in Oncomine Cancer Microarray database. CRC: colorectal carcinoma; GPR116: G protein-coupled receptor 116; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; SE: standard error.

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Western Blot, Microarray

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: Association between GPR116 expression and clinicopathologic features in patients with colorectal carcinoma

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing

Journal: Oncotarget

Article Title: High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma

doi: 10.18632/oncotarget.18203

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic parameters for survival in patients with colorectal carcinoma

Article Snippet: Human colorectal carcinoma cell lines (HCT116, SW1116, SW480 and LOVO) and normal colonic mucosa epithelial cell line (FHC) were purchased from the American Type Culture Collection.

Techniques: Expressing